: Establish The cycle at which the next derivative of fluorescence with regard to cycle range is maximal (Rasmussen, 2001). Regional fitting into a parabola could possibly be used to outline the peak placement with sub‐cycle precision. This is especially a concern when working with tissues substantial in endogenous RNase https://www.facebook.com/NewTechFind/posts/pfbid036eNrLybBWwh8vy1nYnPmDPyphvC767uAeRC9EjLyWQZEaMzEdiet7HMwTVo5JZmZl